On the Formation of Lactic Acid and Carbonic Acid during Muscular Contraction and Rigor Mortis.
نویسنده
چکیده
When living muscular tissue is at rest its reaction to test paper is faintly alkaline or neutral. When it contracts, and during the period of rigor mortis, carbonic acid is set free, and the reaction of the muscle to test paper becomes distinctly acid. This acidity is supposed to result from the liberation of lactic acid ; but in what way this acid and carbonic acid are produced, or from what proteid constituent they arise has not hitherto been determined. Carbonic acid cannot be directly combined with lactic acid CH3 * CH(OH) *COOH; the synthesis, however, may be effected by adopting the following method. Pour ten grammes of pyruvic acid CH3 CO COOH very slowly and gently over some finely powdered potassium cyanide, then add slowly 12 C.C. of concentrated hydrochloric acid; pour the whole into a flask containing a hot solution of twenty grammes of barium hydrate in 250 c.c. of water; attach a reversible condenser and boil for two hours, neglecting any immediate precipitate which may be formed. After cooling collect the white crystalline salt on a filter. This salt, which is barium methyl tartronate, when dried at I00 OC. weighs about i6 grammes and contains only a very small quantity of barium carbonate.1 The following is the reaction
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 3 4 شماره
صفحات -
تاریخ انتشار 2005